Publications
2016
RYBP stimulates PRC1 to shape chromatin-based communication between Polycomb repressive complexes
Rose, N.R., King, H.W., Blackledge, N.P., Fursova, N.A., Ember, K.J., Fischer, R., Kessler, B.M., Klose, R.J. (2016) RYBP stimulates PRC1 to shape chromatin-based communication between Polycomb repressive complexes. elife. 5,e18591
Protection of CpG islands from DNA methylation is DNA-encoded and evolutionarily conserved
Long, H.K., King, H.W., Patient, R.K., Odom, D.T., Klose, R.J. (2016) Protection of CpG islands from DNA methylation is DNA-encoded and evolutionarily conserved. Nucleic Acids Res. 44(14), 6693-6706
2015
Histone demethylases in chromatin biology and beyond
Dimitrova, E., Turberfield, A.H., Klose, R.J. (2015) Histone demethylases in chromatin biology and beyond. EMBO Rep. 16 (12), 1620-39
Targeting Polycomb systems to regulate gene expression: modifications to a complex story
Blackledge, N.P., Rose, N.R., Klose, R.J. (2015) Targeting Polycomb systems to regulate gene expression: modifications to a complex story. Nat Rev Mol Cell Biol. 16 (11), 643-9
2014
Targeting polycomb to pericentric heterochromatin in embryonic stem cells reveals a role for H2AK119u1 in PRC2 recruitment
Cooper, S., Dienstbier, M., Hassan, R., Schermelleh, L., Sharif, J., Blackledge, N.P., De Marco, V., Elderkin, S., Koseki, H., Klose, R., Heger, A., Brockdorff, N. Targeting Polycomb to Pericentric Heterochromatin in Embryonic Stem Cells Reveals a Role for H2AK119u1 in PRC2 Recruitment. Cell Reports. 7 (5), 1456-1470
http://www.sciencedirect.com/science/article/pii/S221112471400299X
Variant PRC1 complex-dependent H2A ubiquitylation drives PRC2 recruitment and polycomb domain formation
Blackledge, N.P., Farcas, A.M., Kondo, T., King, H.W., McGouran, J.F., Hanssen, L.L., Ito, S., Cooper, S., Kondo, K., Koseki, Y., Ishikura, T., Long, H.K., Sheahan, T.W., Brockdorff, N., Kessler, B.M., Koseki, H., Klose, R.J. (2014) Variant PRC1 Complex-Dependent H2A Ubiquitylation Drives PRC2 Recruitment and Polycomb Domain Formation. Cell. 157 (6), 1445-1459
http://www.sciencedirect.com/science/article/pii/S0092867414005893
Understanding the relationship between DNA methylation and histone lysine methylation
Rose, N. R. and Klose R. J. Understanding the relationship between DNA methylation and histone lysine methylation. Biochimica et Biophysica Acta (BBA) – Gene Regulatory Mechanisms. 1839 (12), 1362-1372
http://www.sciencedirect.com/science/article/pii/S1874939914000285
2013
Chromatin sampling–an emerging perspective on targeting polycomb repressor proteins
Klose, R. J., Cooper, S., Farcas, A. M., Blackledge, N. P., and Brockdorff, N. (2013) Chromatin sampling–an emerging perspective on targeting polycomb repressor proteins. PLoS Genet. 9, e1003717
http://www.plosgenetics.org/article/info%3Adoi%2F10.1371%2Fjournal.pgen.1003717
ZF-CxxC domain-containing proteins, CpG islands and the chromatin connection
Long, H. K., Blackledge, N. P. and Klose, R. J. (2013) ZF-CxxC domain-containing proteins, CpG islands and the chromatin connection. Biochem Soc Trans. 41, 727-740
Epigenetic conservation at gene regulatory elements revealed by non-methylated DNA profiling in seven vertebrates
Long, H. K., Sims, D., Heger, A., Blackledge, N. P., Kutter, C., Wright, M. L., Grützner, F., Odom, D. T., Patient, R., Ponting, C. P. and Klose, R. J. (2013) Epigenetic conservation at gene regulatory elements revealed by non-methylated DNA profiling in seven vertebrates. elife. 2, e00348
http://elife.elifesciences.org/content/2/e00348
2012
KDM2B links the Polycomb Repressive Complex 1 (PRC1) to recognition of CpG islands
Farcas, A. M., Blackledge, N. P., Sudbery, I., Long, H. K., McGouran, J. F., Rose, N. R., Lee, S., Sims, D., Cerase, A., Sheahan, T. W., Koseki, H., Brockdorff, N., Ponting, C. P., Kessler, B. M. and Klose, R. J. (2012) KDM2B links the Polycomb Repressive Complex 1 (PRC1) to recognition of CpG islands. elife. 1, e00205
Plant Growth Regulator Daminozide Is a Selective Inhibitor of Human KDM2/7 Histone Demethylases
Rose, N. R., Woon, E. C., Tumber, A., Walport, L. J., Chowdhury, R., Li, X. S., King, O. N., Lejeune, C., Ng, S. S., Krojer, T., Chan, M. C., Rydzik, A. M., Hopkinson, R. J., Che, K. H., Daniel, M., Strain-Damerell, C., Gileadi, C., Kochan, G., Leung, I. K., Dunford, J., Yeoh, K. K., Ratcliffe, P. J., Burgess-Brown, N., von Delft, F., Muller, S., Marsden, B., Brennan, P. E., McDonough, M. A., Oppermann, U., Klose, R. J., Schofield, C. J. and Kawamura, A. (2012) Plant growth regulator daminozide is a selective inhibitor of human KDM2/7 histone demethylases. J Med Chem. 55, 6639-6643
Bio-CAP: a versatile and highly sensitive technique to purify and characterise regions of non-methylated DNA
Blackledge, N. P., Long, H. K., Zhou, J. C., Kriaucionis, S., Patient, R. and Klose, R. J. (2012) Bio-CAP: a versatile and highly sensitive technique to purify and characterise regions of non-methylated DNA. Nucleic Acids Res. 40, e32
2011
Recognition of CpG Island Chromatin by KDM2A Requires Direct and Specific Interaction with Linker DNA
Zhou, J. C., Blackledge, N. P., Farcas, A. M. andKlose, R. J. (2012) Recognition of CpG island chromatin by KDM2A requires direct and specific interaction with linker DNA. Mol Cell Biol. 32, 479-489
The oncometabolite 2-hydroxyglutarate inhibits histone lysine demethylases
Chowdhury, R., Yeoh, K. K., Tian, Y. M., Hillringhaus, L., Bagg, E. A., Rose, N. R., Leung, I. K., Li, X. S., Woon, E. C., Yang, M., McDonough, M. A., King, O. N., Clifton, I. J., Klose, R. J., Claridge, T. D., Ratcliffe, P. J., Schofield, C. J. and Kawamura, A. (2011) The oncometabolite 2-hydroxyglutarate inhibits histone lysine demethylases. EMBO Rep. 12, 463-469
2010
Quantitative High-Throughput Screening Identifies 8-Hydroxyquinolines as Cell-Active Histone Demethylase Inhibitors
King, O. N., Li, X. S., Sakurai, M., Kawamura, A., Rose, N. R., Ng, S. S., Quinn, A. M., Rai, G., Mott, B. T., Beswick, P., Klose, R. J., Oppermann, U., Jadhav, A., Heightman, T. D., Maloney, D. J., Schofield, C. J. and Simeonov, A. (2010) Quantitative high-throughput screening identifies 8-hydroxyquinolines as cell-active histone demethylase inhibitors. PLoS One. 5, e15535
http://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0015535
Jarid2 is a PRC2 component in embryonic stem cells required for multi-lineage differentiation and recruitment of PRC1 and RNA Polymerase II to developmental regulators
Landeira, D., Sauer, S., Poot, R., Dvorkina, M., Mazzarella, L., Jorgensen, H. F., Pereira, C. F., Leleu, M., Piccolo, F. M., Spivakov, M., Brookes, E., Pombo, A., Fisher, C., Skarnes, W. C., Snoek, T., Bezstarosti, K., Demmers, J., Klose, R. J., Casanova, M., Tavares, L., Brockdorff, N., Merkenschlager, M. and Fisher, A. G. (2010) Jarid2 is a PRC2 component in embryonic stem cells required for multi-lineage differentiation and recruitment of PRC1 and RNA Polymerase II to developmental regulators. Nat Cell Biol. 12, 618-624
CpG islands recruit a histone H3 lysine 36 demethylase
Blackledge, N. P., Zhou, J. C., Tolstorukov, M. Y., Farcas, A. M., Park, P. J. and Klose, R. J. (2010) CpG islands recruit a histone H3 lysine 36 demethylase. Mol Cell. 38, 179-190
http://www.sciencedirect.com/science/article/pii/S1097276510002868
Histone lysine methylation: an epigenetic modification?
Blackledge, N. P. and Klose, R. J. (2010) Histone lysine methylation: an epigenetic modification? Epigenomics. 2, 151-161
PHF8, a gene associated with cleft lip/palate and mental retardation, encodes for an Nε-dimethyl lysine demethylase
Loenarz, C., Ge, W., Coleman, M. L., Rose, N. R., Cooper, C. D., Klose, R. J., Ratcliffe, P. J. and Schofield, C. J. (2010) PHF8, a gene associated with cleft lip/palate and mental retardation, encodes for an Nepsilon-dimethyl lysine demethylase. Hum Mol Genet. 19, 217-222
2009
Phosphorylation of MeCP2 at Serine 80 regulates its chromatin association and neurological function
Tao, J., Hu, K., Chang, Q., Wu, H., Sherman, N. E., Martinowich, K., Klose, R. J., Schanen, C., Jaenisch, R., Wang, W. and Sun, Y. E. (2009) Phosphorylation of MeCP2 at Serine 80 regulates its chromatin association and neurological function.Proc Natl Acad Sci U S A. 106, 4882-4887
2008
Dynamic protein methylation in chromatin biology
Ng, S. S., Yue, W. W., Oppermann, U. and Klose, R. J. (2009) Dynamic protein methylation in chromatin biology. Cell Mol Life Sci. 66, 407-422
http://link.springer.com/article/10.1007%2Fs00018-008-8303-z
MeCP2 Binding to DNA Depends upon Hydration at Methyl-CpG
Ho, K. L., McNae, I. W., Schmiedeberg, L., Klose, R. J., Bird, A. P. and Walkinshaw, M. D. (2008) MeCP2 binding to DNA depends upon hydration at methyl-CpG. Mol Cell. 29, 525-531
http://www.sciencedirect.com/science/article/pii/S1097276508000968
2007
Histone H3 Arg2 methylation provides alternative directions for COMPASS
Klose, R. J. and Zhang, Y. (2007) Histone H3 Arg2 methylation provides alternative directions for COMPASS. Nat Struct Mol Biol. 14, 1058-1060
http://www.nature.com/nsmb/journal/v14/n11/full/nsmb1107-1058.html
Demethylation of Histone H3K36 and H3K9 by Rph1: a Vestige of an H3K9 Methylation System in Saccharomyces cerevisiae?
Klose, R. J., Gardner, K. E., Liang, G., Erdjument-Bromage, H., Tempst, P. and Zhang, Y. (2007) Demethylation of histone H3K36 and H3K9 by Rph1: a vestige of an H3K9 methylation system in Saccharomyces cerevisiae? Mol Cell Biol. 27, 3951-3961