Fursova NA, Blackledge NP, Nakayama M, Ito S, Koseki Y, Farcas AM, King HW, Koseki H, Klose RJ. (2019) Synergy between Variant PRC1 Complexes Defines Polycomb-Mediated Gene Repression.

Mol Cell. 2019 Jun 6;74(5):1020-1036.e8. doi: 10.1016/j.molcel.2019.03.024. 


Turberfield AH, Kondo T, Nakayama M, Koseki Y, King HW, Koseki H, Klose RJ.( 2019) KDM2 proteins constrain transcription from CpG island gene promoters independently of their histone demethylase activity.

Nucleic Acids Res. 2019 Sep 26;47(17):9005-9023. doi: 10.1093/nar/gkz607.



King H.W., Fursova N.A., Blackledge N.P., Klose R.J. (2018) Polycomb repressive complex 1 shapes the nucleosome landscape but not accessibility at target genes. Genome Res. 28(10):1494-1507.

Dimitrova E., Kondo T., Feldmann A., Nakayama M., Koseki Y., Konietzny R., Kessler B.M., Koseki H., Klose R.J. (2018) FBXL19 recruits CDK-Mediator to CpG islands of developmental genes priming them for activation during lineage commitment. elife. 7,e37084

Long H.K., Rose N.R., Blackledge N.P., Klose R.J. (2018) Biochemical Identification of Nonmethylated DNA by BioCAP-Seq. Methods Mol Biol. 1766:15-29


Brown D.A., Di Cerbo V., Feldmann A., Ahn J., Ito S., Blackledge N.P., Nakayama M., McClellan M., Dimitrova E., Turberfield A.H., Long H.K., King H.W., Kriaucionis S., Schermelleh L., Kutateladze T.G., Koseki H., Klose R.J. (2017) The SET1 Complex Selects Actively Transcribed Target Genes via Multivalent Interaction with CpG Island Chromatin. Cell Rep. 20(10):2313-2327

Endoh, M., Endo, T.A., Shinga, J., Hayashi, K., Farcas, A., Ma, K.W., Ito, S., Sharif, J., Endoh, T., Onaga, N., Nakayama, M., Ishikura, T., Masui, O., Kessler, B.M., Suda, T., Ohara, O., Okuda, A., Klose, R., Koseki, H. (2017) PCGF6-PRC1 suppresses premature differentiation of mouse embryonic stem cells by regulating germ cell-related genes. elife. 6,e21064

King, H.W., Klose, R.J. (2017) The pioneer factor OCT4 requires the chromatin remodeller BRG1 to support gene regulatory element function in mouse embryonic stem cells. elife. 6,e22631

Kerry, J., Godfrey, L., Repapi, E., Tapia, M., Blackledge, N.P., Ma, H., Ballabio, E., O’Byrne, S., Ponthan, F., Heidenreich, O., Roy, A., Roberts, I., Konopleva, M., Klose, R.J., Geng, H., Milne, T.A. (2017) MLL-AF4 Spreading Identifies Binding Sites that Are Distinct from Super-Enhancers and that Govern Sensitivity to DOT1L Inhibition in Leukemia. Cell Rep. 18(2),482-495


Ernst, C., Pike, J., Aitken, S.J., Long, H.K., Eling, N., Stojic, L., Ward, M.C., Connor, F., Rayner, T.F., Lukk, M., Klose, R.J., Kutter, C., Odom, D.T. (2016) Successful transmission and transcriptional deployment of a human chromosome via mouse male meiosis. elife. 5,e20235

Rose, N.R., King, H.W., Blackledge, N.P., Fursova, N.A., Ember, K.J., Fischer, R., Kessler, B.M., Klose, R.J. (2016) RYBP stimulates PRC1 to shape chromatin-based communication between Polycomb repressive complexes. elife. 5,e18591

Long, H.K., King, H.W., Patient, R.K., Odom, D.T., Klose, R.J. (2016) Protection of CpG islands from DNA methylation is DNA-encoded and evolutionarily conserved. Nucleic Acids Res. 44(14), 6693-6706


Dimitrova, E., Turberfield, A.H., Klose, R.J. (2015) Histone demethylases in chromatin biology and beyond. EMBO Rep. 16 (12), 1620-39

Blackledge, N.P., Rose, N.R., Klose, R.J. (2015) Targeting Polycomb systems to regulate gene expression: modifications to a complex story. Nat Rev Mol Cell Biol. 16 (11), 643-9


Blackledge, N.P., Farcas, A.M., Kondo, T., King, H.W., McGouran, J.F., Hanssen, L.L., Ito, S., Cooper, S., Kondo, K., Koseki, Y., Ishikura, T., Long, H.K., Sheahan, T.W., Brockdorff, N., Kessler, B.M., Koseki, H., Klose, R.J. (2014) Variant PRC1 Complex-Dependent H2A Ubiquitylation Drives PRC2 Recruitment and Polycomb Domain Formation. Cell. 157 (6), 1445-1459

Cooper, S., Dienstbier, M., Hassan, R., Schermelleh, L., Sharif, J., Blackledge, N.P., De Marco, V., Elderkin, S., Koseki, H., Klose, R., Heger, A., Brockdorff, N. Targeting Polycomb to Pericentric Heterochromatin in Embryonic Stem Cells Reveals a Role for H2AK119u1 in PRC2 Recruitment. Cell Reports. 7 (5), 1456-1470

Rose, N. R. and Klose R. J. Understanding the relationship between DNA methylation and histone lysine methylation. Biochimica et Biophysica Acta (BBA) – Gene Regulatory Mechanisms. 1839 (12), 1362-1372


Klose, R. J., Cooper, S., Farcas, A. M., Blackledge, N. P., and Brockdorff, N. (2013) Chromatin sampling–an emerging perspective on targeting polycomb repressor proteins. PLoS Genet. 9, e1003717

Long, H. K., Blackledge, N. P. and Klose, R. J. (2013) ZF-CxxC domain-containing proteins, CpG islands and the chromatin connection. Biochem Soc Trans. 41, 727-740

Long, H. K., Sims, D., Heger, A., Blackledge, N. P., Kutter, C., Wright, M. L., Gr├╝tzner, F., Odom, D. T., Patient, R., Ponting, C. P. and Klose, R. J. (2013) Epigenetic conservation at gene regulatory elements revealed by non-methylated DNA profiling in seven vertebrates. elife. 2, e00348


Farcas, A. M., Blackledge, N. P., Sudbery, I., Long, H. K., McGouran, J. F., Rose, N. R., Lee, S., Sims, D., Cerase, A., Sheahan, T. W., Koseki, H., Brockdorff, N., Ponting, C. P., Kessler, B. M. and Klose, R. J. (2012) KDM2B links the Polycomb Repressive Complex 1 (PRC1) to recognition of CpG islands. elife. 1, e00205

Rose, N. R., Woon, E. C., Tumber, A., Walport, L. J., Chowdhury, R., Li, X. S., King, O. N., Lejeune, C., Ng, S. S., Krojer, T., Chan, M. C., Rydzik, A. M., Hopkinson, R. J., Che, K. H., Daniel, M., Strain-Damerell, C., Gileadi, C., Kochan, G., Leung, I. K., Dunford, J., Yeoh, K. K., Ratcliffe, P. J., Burgess-Brown, N., von Delft, F., Muller, S., Marsden, B., Brennan, P. E., McDonough, M. A., Oppermann, U., Klose, R. J., Schofield, C. J. and Kawamura, A. (2012) Plant growth regulator daminozide is a selective inhibitor of human KDM2/7 histone demethylases. J Med Chem. 55, 6639-6643

Blackledge, N. P., Long, H. K., Zhou, J. C., Kriaucionis, S., Patient, R. and Klose, R. J. (2012) Bio-CAP: a versatile and highly sensitive technique to purify and characterise regions of non-methylated DNA. Nucleic Acids Res. 40, e32

Zhou, J. C., Blackledge, N. P., Farcas, A. M. andKlose, R. J. (2012) Recognition of CpG island chromatin by KDM2A requires direct and specific interaction with linker DNA. Mol Cell Biol. 32, 479-489


Chowdhury, R., Yeoh, K. K., Tian, Y. M., Hillringhaus, L., Bagg, E. A., Rose, N. R., Leung, I. K., Li, X. S., Woon, E. C., Yang, M., McDonough, M. A., King, O. N., Clifton, I. J., Klose, R. J., Claridge, T. D., Ratcliffe, P. J., Schofield, C. J. and Kawamura, A. (2011) The oncometabolite 2-hydroxyglutarate inhibits histone lysine demethylases. EMBO Rep. 12, 463-469


King, O. N., Li, X. S., Sakurai, M., Kawamura, A., Rose, N. R., Ng, S. S., Quinn, A. M., Rai, G., Mott, B. T., Beswick, P., Klose, R. J., Oppermann, U., Jadhav, A., Heightman, T. D., Maloney, D. J., Schofield, C. J. and Simeonov, A. (2010) Quantitative high-throughput screening identifies 8-hydroxyquinolines as cell-active histone demethylase inhibitors. PLoS One. 5, e15535

Landeira, D., Sauer, S., Poot, R., Dvorkina, M., Mazzarella, L., Jorgensen, H. F., Pereira, C. F., Leleu, M., Piccolo, F. M., Spivakov, M., Brookes, E., Pombo, A., Fisher, C., Skarnes, W. C., Snoek, T., Bezstarosti, K., Demmers, J., Klose, R. J., Casanova, M., Tavares, L., Brockdorff, N., Merkenschlager, M. and Fisher, A. G. (2010) Jarid2 is a PRC2 component in embryonic stem cells required for multi-lineage differentiation and recruitment of PRC1 and RNA Polymerase II to developmental regulators. Nat Cell Biol. 12, 618-624

Blackledge, N. P., Zhou, J. C., Tolstorukov, M. Y., Farcas, A. M., Park, P. J. and Klose, R. J. (2010) CpG islands recruit a histone H3 lysine 36 demethylase. Mol Cell. 38, 179-190

Blackledge, N. P. and Klose, R. J. (2010) Histone lysine methylation: an epigenetic modification?Epigenomics. 2, 151-161

Loenarz, C., Ge, W., Coleman, M. L., Rose, N. R., Cooper, C. D., Klose, R. J., Ratcliffe, P. J. and Schofield, C. J. (2010) PHF8, a gene associated with cleft lip/palate and mental retardation, encodes for an Nepsilon-dimethyl lysine demethylase. Hum Mol Genet. 19, 217-222


Tao, J., Hu, K., Chang, Q., Wu, H., Sherman, N. E., Martinowich, K., Klose, R. J., Schanen, C., Jaenisch, R., Wang, W. and Sun, Y. E. (2009) Phosphorylation of MeCP2 at Serine 80 regulates its chromatin association and neurological function.Proc Natl Acad Sci U S A. 106, 4882-4887

Ng, S. S., Yue, W. W., Oppermann, U. and Klose, R. J. (2009) Dynamic protein methylation in chromatin biology. Cell Mol Life Sci. 66, 407-422


Ho, K. L., McNae, I. W., Schmiedeberg, L., Klose, R. J., Bird, A. P. and Walkinshaw, M. D. (2008) MeCP2 binding to DNA depends upon hydration at methyl-CpG. Mol Cell. 29, 525-531

Before Oxford


Klose, R. J. and Zhang, Y. (2007) Histone H3 Arg2 methylation provides alternative directions for COMPASS. Nat Struct Mol Biol. 14, 1058-1060

Klose, R. J., Gardner, K. E., Liang, G., Erdjument-Bromage, H., Tempst, P. and Zhang, Y. (2007) Demethylation of histone H3K36 and H3K9 by Rph1: a vestige of an H3K9 methylation system in Saccharomyces cerevisiae? Mol Cell Biol. 27, 3951-3961

Yamane, K., Tateishi, K., Klose, R. J., Fang, J., Fabrizio, L. A., Erdjument-Bromage, H., Taylor-Papadimitriou, J., Tempst, P. and Zhang, Y. (2007) PLU-1 is an H3K4 demethylase involved in transcriptional repression and breast cancer cell proliferation. Mol Cell. 25, 801-812

Lee, N., Zhang, J., Klose, R. J., Erdjument-Bromage, H., Tempst, P., Jones, R. S. and Zhang, Y. (2007) The trithorax-group protein Lid is a histone H3 trimethyl-Lys4 demethylase. Nat Struct Mol Biol. 14, 341-343

Klose, R. J. and Zhang, Y. (2007) Regulation of histone methylation by demethylimination and demethylation. Nat Rev Mol Cell Biol. 8, 307-318

Klose, R. J., Yan, Q., Tothova, Z., Yamane, K., Erdjument-Bromage, H., Tempst, P., Gilliland, D. G., Zhang, Y. and Kaelin, W. G., Jr. (2007) The retinoblastoma binding protein RBP2 is an H3K4 demethylase. Cell. 128, 889-900

Liang, G., Klose, R. J., Gardner, K. E. and Zhang, Y. (2007) Yeast Jhd2p is a histone H3 Lys4 trimethyl demethylase. Nat Struct Mol Biol. 14, 243-245


Klose, R. J., Kallin, E. M. and Zhang, Y. (2006) JmjC-domain-containing proteins and histone demethylation. Nat Rev Genet. 7, 715-727

Klose, R. J., Yamane, K., Bae, Y., Zhang, D., Erdjument-Bromage, H., Tempst, P., Wong, J. and Zhang, Y. (2006) The transcriptional repressor JHDM3A demethylates trimethyl histone H3 lysine 9 and lysine 36. Nature. 442, 312-316

Klose, R. J. and Bird, A. P. (2006) Genomic DNA methylation: the mark and its mediators. Trends Biochem Sci. 31, 89-97


Klose, R. J., Sarraf, S. A., Schmiedeberg, L., McDermott, S. M., Stancheva, I. and Bird, A. P. (2005) DNA binding selectivity of MeCP2 due to a requirement for A/T sequences adjacent to methyl-CpG. Mol Cell. 19, 667-678


Klose, R. J. and Bird, A. P. (2004) MeCP2 behaves as an elongated monomer that does not stably associate with the Sin3a chromatin remodeling complex. J Biol Chem. 279, 46490-46496


Li, X., Barrett, J., Pang, A., Klose, R. J., Krell, P. J. and Arif, B. M. (2000) Characterization of an overexpressed spindle protein during a baculovirus infection. Virology. 268, 56-67