Paper celebration
Great to celebrate a recent paper acceptance with the team and collagues on the floor!
The Polycomb system sustains promoters in a deep OFF state by limiting pre-initiation complex formation to counteract transcription
Szczurek AT, Dimitrova E, Kelley JR, Blackledge NP, Klose RJ.
Nature Cell Biology 2024
Extensive DNA methylome rearrangement during early lamprey embryogenesis.
Angeloni A, Fissette S, Kaya D, Hammond JM, Gamaarachchi H, Deveson IW, Klose RJ, Li W, Zhang X, Bogdanovic O.
Nat Communication 2024.
Fun making competent bugs!
Competent bug making party!
Congratulations to Dr Au!
Recycling of modified H2A-H2B provides short-term memory of chromatin states
Flury V, Reverón-Gómez N, Alcaraz N, Stewart-Morgan KR, Wenger A, Klose RJ, Groth A. Cell. 2023 Mar 2;186(5):1050-1065.e19. Epub 2023 Feb 6.
PMID: 36750094; PMCID: PMC9994263.
doi: 10.1016/j.cell.2023.01.007.
A CpG island-encoded mechanism protects genes from premature transcription termination
Hughes AL, Szczurek AT, Kelley JR, Lastuvkova A, Turberfield AH, Dimitrova E, Blackledge NP, Klose RJ.
Nat Commun. 2023 Feb 9;14(1):726.
doi: 10.1038/s41467-023-36236-2. PMID: 36759609; PMCID: PMC9911701.
Changes in PRC1 activity during interphase modulate lineage transition in pluripotent cells
Asenjo HG, Alcazar-Fabra M, Espinosa-Martínez M, Lopez-Onieva L, Gallardo A, Dimitrova E, Feldmann A, Pachano T, Martorell-Marugán J, Carmona-Sáez P, Sanchez-Pozo A, Rada-Iglesias Á, Klose RJ, Landeira D. Nat Commun. 2023 Jan 12;14(1):180. PMID: 36635295; PMCID: PMC9837203.
doi: 10.1038/s41467-023-35859-9.
PCGF1-PRC1 links chromatin repression with DNA replication during hematopoietic cell lineage commitment
Takano J, Ito S, Dong Y, Sharif J, Nakajima-Takagi Y, Umeyama T, Han YW, Isono K, Kondo T, Iizuka Y, Miyai T, Koseki Y, Ikegaya M, Sakihara M, Nakayama M, Ohara O, Hasegawa Y, Hashimoto K, Arner E, Klose RJ, Iwama A, Koseki H, Ikawa T. Nat Commun. 2022 Nov 28;13(1):7159.
PMID: 36443290; PMCID: PMC9705430.
doi: 10.1038/s41467-022-34856-8.
Distinct roles for CKM-Mediator in controlling Polycomb-dependent chromosomal interactions and priming genes for induction
Dimitrova E, Feldmann A, van der Weide RH, Flach KD, Lastuvkova A, de Wit E, Klose RJ. Nat Struct Mol Biol. 2022 Oct;29(10):1000-1010.
doi: 10.1038/s41594-022-00840-5.
Epub 2022 Oct 11. PMID: 36220895; PMCID: PMC9568430.
The molecular principles of gene regulation by Polycomb repressive complexes
Blackledge NP, Klose RJ.
Nat Rev Mol Cell Biol. 2021 Dec;22(12):815-833.
doi: 10.1038/s41580-021-00398-y.
Epub 2021 Aug 16. PMID: 34400841; PMCID: PMC7612013.
PRC1 drives Polycomb-mediated gene repression by controlling transcription initiation and burst frequency
Dobrinić, P., Szczurek, A.T. & Klose, R.J
Nat Struct Mol Biol 28, 811–824 (2021).
https://doi.org/10.1038/s41594-021-00661-y
Getting under the skin of Polycomb-dependent gene regulation
Neil P. Blackledge and Robert J. Klose
doi:10.1101/gad.348257.121Genes & Dev. 2021. 35: 301-303
Congratulations Dr Dobrinic and Dr Szczurek
on their recent publication PRC1 drives Polycomb-mediated gene repression by controlling transcription initiation and burst frequency
Variant PCGF1-PRC1 links PRC2 recruitment with differentiation-associated transcriptional inactivation at target genes
Sugishita H, Kondo T, Ito S, Nakayama M, Yakushiji-Kaminatsui N, Kawakami E, Koseki Y, Ohinata Y, Sharif J, Harachi M, Blackledge NP, Klose RJ, Koseki H. Nat Commun. 2021 Sep 9;12(1):5341.
doi: 10.1038/s41467-021-24894-z.
PMID: 34504070; PMCID: PMC8429492.
Congratulations to Dr Amy Hughes for sucessfully defending her PhD
BAP1 constrains pervasive H2AK119ub1 to control the transcriptional potential of the genome
Nadezda A. Fursova, Anne H. Turberfield, Neil P. Blackledge, Emma L. Findlater, Anna Lastuvkova, Miles K. Huseyin, Paula Dobrinić, Robert J. Klose
Genes Dev. 2021 May 1;35(9-10):749-770. Epub 2021 Apr 22.
doi: 10.1101/gad.347005.120.
PMID: 33888563; PMCID: PMC8091973.
Live-cell single particle tracking of PRC1 reveals a highly dynamic system with low target site occupancy
Huseyin, M.K., Klose, R
Nat Commun 12, 887 (2021).
https://doi.org/10.1038/s41467-021-21130-6
Distinct contributions of DNA methylation and histone acetylation to the genomic occupancy of transcription factors
Cusack M, King HW, Spingardi P, Kessler BM, Klose RJ, Kriaucionis S. Distinct contributions of DNA methylation and histone acetylation to the genomic occupancy of transcription factors. Genome Res. 2020 Oct;30(10):1393-1406. doi: 10.1101/gr.257576.119. Epub 2020 Sep 22. PMID: 32963030; PMCID: PMC7605266.
PMID: 32963030
PMCID: PMC7605266
PRC1 drives Polycomb-mediated gene repression by controlling transcription initiation and burst frequency
Paula Dobrinić, Aleksander T. Szczurek, Robert J. Klose
bioRxiv 2020.10.09.333294; doi: https://doi.org/10.1101/2020.10.09.333294
Understanding the interplay between CpG island-associated gene promoters and H3K4 methylation
Hughes AL, Kelley JR, Klose RJ. Understanding the interplay between CpG island-associated gene promoters and H3K4 methylation. Biochim Biophys Acta Gene Regul Mech. 2020 Aug;1863(8):194567. doi: 10.1016/j.bbagrm.2020.194567. Epub 2020 Apr 29. PMID: 32360393; PMCID: PMC7294231.
PMID: 32360393
PMCID: PMC7294231
Identifying proteins bound to native mitotic ESC chromosomes reveals chromatin repressors are important for compaction
Djeghloul D, Patel B, Kramer H, Dimond A, Whilding C, Brown K, Kohler AC, Feytout A, Veland N, Elliott J, Bharat TAM, Tarafder AK, Löwe J, Ng BL, Guo Y, Guy J, Huseyin MK, Klose RJ, Merkenschlager M, Fisher AG. Identifying proteins bound to native mitotic ESC chromosomes reveals chromatin repressors are important for compaction. Nat Commun. 2020 Aug 17;11(1):4118. doi: 10.1038/s41467-020-17823-z. PMID: 32807789; PMCID: PMC7431861.
PMID: 32807789
PMCID: PMC7431861
Analysis of Genome Architecture during SCNT Reveals a Role of Cohesin in Impeding Minor ZGA
Zhang K, Wu DY, Zheng H, Wang Y, Sun QR, Liu X, Wang LY, Xiong WJ, Wang Q, Rhodes JDP, Xu K, Li L, Lin Z, Yu G, Xia W, Huang B, Du Z, Yao Y, Nasmyth KA, Klose RJ, Miao YL, Xie W. Analysis of Genome Architecture during SCNT Reveals a Role of Cohesin in Impeding Minor ZGA. Mol Cell. 2020 Jul 16;79(2):234-250.e9. doi: 10.1016/j.molcel.2020.06.001. Epub 2020 Jun 23. PMID: 32579944.
PMID: 32579944
Live-cell single particle tracking of PRC1 reveals a highly dynamic system with low target site occupancy
Miles K. Huseyin, Robert J. Klose
bioRxiv 2020.04.25.061358; doi: https://doi.org/10.1101/2020.04.25.061358