Neil P. Blackledge and Robert J. Klose

doi:10.1101/gad.348257.121Genes & Dev. 2021. 35: 301-303

Dobrinić, P., Szczurek, A.T. & Klose, R.J. PRC1 drives Polycomb-mediated gene repression by controlling transcription initiation and burst frequency. Nat Struct Mol Biol 28, 811–824 (2021). https://doi.org/10.1038/s41594-021-00661-y

Huseyin, M.K., Klose, R.J. Live-cell single particle tracking of PRC1 reveals a highly dynamic system with low target site occupancy. Nat Commun 12, 887 (2021).

https://doi.org/10.1038/s41467-021-21130-6

Nadezda A. Fursova, Anne H. Turberfield, Neil P. Blackledge, Emma L. Findlater, Anna Lastuvkova, Miles K. Huseyin, Paula Dobrinić, Robert J. Klose

bioRxiv 2020.11.13.381251; doi: https://doi.org/10.1101/2020.11.13.381251

Cusack M, King HW, Spingardi P, Kessler BM, Klose RJ, Kriaucionis S. Distinct contributions of DNA methylation and histone acetylation to the genomic occupancy of transcription factors. Genome Res. 2020 Oct;30(10):1393-1406. doi: 10.1101/gr.257576.119. Epub 2020 Sep 22. PMID: 32963030; PMCID: PMC7605266.

PMID: 32963030

PMCID: PMC7605266

DOI: 10.1101/gr.257576.119

Paula Dobrinić, Aleksander T. Szczurek, Robert J. Klose

bioRxiv 2020.10.09.333294; doi: https://doi.org/10.1101/2020.10.09.333294

Hughes AL, Kelley JR, Klose RJ. Understanding the interplay between CpG island-associated gene promoters and H3K4 methylation. Biochim Biophys Acta Gene Regul Mech. 2020 Aug;1863(8):194567. doi: 10.1016/j.bbagrm.2020.194567. Epub 2020 Apr 29. PMID: 32360393; PMCID: PMC7294231.

PMID: 32360393

PMCID: PMC7294231

DOI: 10.1016/j.bbagrm.2020.194567

Djeghloul D, Patel B, Kramer H, Dimond A, Whilding C, Brown K, Kohler AC, Feytout A, Veland N, Elliott J, Bharat TAM, Tarafder AK, Löwe J, Ng BL, Guo Y, Guy J, Huseyin MK, Klose RJ, Merkenschlager M, Fisher AG. Identifying proteins bound to native mitotic ESC chromosomes reveals chromatin repressors are important for compaction. Nat Commun. 2020 Aug 17;11(1):4118. doi: 10.1038/s41467-020-17823-z. PMID: 32807789; PMCID: PMC7431861.

PMID: 32807789

PMCID: PMC7431861

DOI: 10.1038/s41467-020-17823-z

Zhang K, Wu DY, Zheng H, Wang Y, Sun QR, Liu X, Wang LY, Xiong WJ, Wang Q, Rhodes JDP, Xu K, Li L, Lin Z, Yu G, Xia W, Huang B, Du Z, Yao Y, Nasmyth KA, Klose RJ, Miao YL, Xie W. Analysis of Genome Architecture during SCNT Reveals a Role of Cohesin in Impeding Minor ZGA. Mol Cell. 2020 Jul 16;79(2):234-250.e9. doi: 10.1016/j.molcel.2020.06.001. Epub 2020 Jun 23. PMID: 32579944.

PMID: 32579944

DOI: 10.1016/j.molcel.2020.06.001

Miles K. Huseyin, Robert J. Klose

bioRxiv 2020.04.25.061358; doi: https://doi.org/10.1101/2020.04.25.061358

Feldmann A, Dimitrova E, Kenney A, Lastuvkova A, Klose RJ. CDK-Mediator and FBXL19 prime developmental genes for activation by promoting atypical regulatory interactions. Nucleic Acids Res. 2020 Apr 6;48(6):2942-2955. doi: 10.1093/nar/gkaa064. PMID: 31996894; PMCID: PMC7102981.

PMID: 31996894

PMCID: PMC7102981

DOI: 10.1093/nar/gkaa064

Kriaucionis S, Klose RJ. ATACing DNA Methylation during Differentiation. Mol Cell. 2020 Mar 19;77(6):1159-1161. doi: 10.1016/j.molcel.2020.02.026. PMID: 32200796.

PMID: 32200796

DOI: 10.1016/j.molcel.2020.02.026

Blackledge NP, Fursova NA, Kelley JR, Huseyin MK, Feldmann A, Klose RJ. PRC1 Catalytic Activity Is Central to Polycomb System Function. Mol Cell. 2020 Feb 20;77(4):857-874.e9. doi: 10.1016/j.molcel.2019.12.001. Epub 2019 Dec 27. PMID: 31883950; PMCID: PMC7033600.

PMID: 31883950

PMCID: PMC7033600

DOI: 10.1016/j.molcel.2019.12.001

Rhodes JDP, Feldmann A, Hernández-Rodríguez B, Díaz N, Brown JM, Fursova NA, Blackledge NP, Prathapan P, Dobrinic P, Huseyin MK, Szczurek A, Kruse K, Nasmyth KA, Buckle VJ, Vaquerizas JM, Klose RJ. Cohesin Disrupts Polycomb-Dependent Chromosome Interactions in Embryonic Stem Cells. Cell Rep. 2020 Jan 21;30(3):820-835.e10. doi: 10.1016/j.celrep.2019.12.057. PMID: 31968256; PMCID: PMC6988126.

PMID: 31968256

PMCID: PMC6988126

DOI: 10.1016/j.celrep.2019.12.057

Turberfield AH, Kondo T, Nakayama M, Koseki Y, King HW, Koseki H, Klose RJ.( 2019) KDM2 proteins constrain transcription from CpG island gene promoters independently of their histone demethylase activity.

Nucleic Acids Res. 2019 Sep 26;47(17):9005-9023. doi: 10.1093/nar/gkz607.

PMID:31363749

https://www.ncbi.nlm.nih.gov/pubmed/31363749

Fursova NA, Blackledge NP, Nakayama M, Ito S, Koseki Y, Farcas AM, King HW, Koseki H, Klose RJ. (2019) Synergy between Variant PRC1 Complexes Defines Polycomb-Mediated Gene Repression.

Mol Cell. 2019 Jun 6;74(5):1020-1036.e8. doi: 10.1016/j.molcel.2019.03.024. 

PMID:31029541

https://www.ncbi.nlm.nih.gov/pubmed/31029541

King H.W., Fursova N.A., Blackledge N.P., Klose R.J. (2018) Polycomb repressive complex 1 shapes the nucleosome landscape but not accessibility at target genes. Genome Res. 28(10):1494-1507.

https://www.ncbi.nlm.nih.gov/pubmed/30154222

https://genome.cshlp.org/content/28/10/1494.long

Kulkarni A, Taylor GP, Klose RJ, Schofield CJ, Bangham CR. Histone H2A monoubiquitylation and p38-MAPKs regulate immediate-early gene-like reactivation of latent retrovirus HTLV-1. JCI Insight. 2018 Oct 18;3(20):e123196. doi: 10.1172/jci.insight.123196. PMID: 30333309; PMCID: PMC6237452.

PMID: 30333309

PMCID: PMC6237452

DOI: 10.1172/jci.insight.123196

Dimitrova E., Kondo T., Feldmann A., Nakayama M., Koseki Y., Konietzny R., Kessler B.M., Koseki H., Klose R.J. (2018) FBXL19 recruits CDK-Mediator to CpG islands of developmental genes priming them for activation during lineage commitment. elife. 7,e37084

https://www.ncbi.nlm.nih.gov/pubmed/29809150

https://elifesciences.org/articles/37084

Long H.K., Rose N.R., Blackledge N.P., Klose R.J. (2018) Biochemical Identification of Nonmethylated DNA by BioCAP-Seq. Methods Mol Biol. 1766:15-29

https://www.ncbi.nlm.nih.gov/pubmed/29605845