Publication – ZF-CxxC domain-containing proteins, CpG islands and the chromatin connection
Congratulations to Hannah and Neil for their latest review article.
Read the article here:
Dinner at Exeter College May 2013
Baby James visits the lab – congratulations Neil and Sarah!
Publication – Epigenetic conservation at gene regulatory elements revealed by non-methylated DNA profiling in seven vertebrates
Congratulations to Hannah for her publication in eLife today. Check out the paper here:
Celebrating Anca and Hannah’s eLife paper
Today we celebrated Anca and Hannah’s eLife papers with a glass or two of champagne. Well done Anca and Hannah!
Epigenetic conservation at gene regulatory elements revealed by non-methylated DNA profiling in seven vertebrates
Long, H. K., Sims, D., Heger, A., Blackledge, N. P., Kutter, C., Wright, M. L., Grützner, F., Odom, D. T., Patient, R., Ponting, C. P. and Klose, R. J. (2013) Epigenetic conservation at gene regulatory elements revealed by non-methylated DNA profiling in seven vertebrates. elife. 2, e00348
http://elife.elifesciences.org/content/2/e00348
Celebrating eLife success Jan 2013
Christmas Dinner 2012 – St John’s College
KDM2B links the Polycomb Repressive Complex 1 (PRC1) to recognition of CpG islands
Farcas, A. M., Blackledge, N. P., Sudbery, I., Long, H. K., McGouran, J. F., Rose, N. R., Lee, S., Sims, D., Cerase, A., Sheahan, T. W., Koseki, H., Brockdorff, N., Ponting, C. P., Kessler, B. M. and Klose, R. J. (2012) KDM2B links the Polycomb Repressive Complex 1 (PRC1) to recognition of CpG islands. elife. 1, e00205
Jin successfully defended his DPhil thesis
The first DPhil student from the Klose lab, Jin Zhou, defneded his thesis today. Congratulations!
Publication – KDM2B links the Polycomb Repressive Complex 1 (PRC1) to recognition of CpG islands
Anca’s paper was published in eLife. Congratulations to her and all other co-authors!
Movember 2012
Plant Growth Regulator Daminozide Is a Selective Inhibitor of Human KDM2/7 Histone Demethylases
Rose, N. R., Woon, E. C., Tumber, A., Walport, L. J., Chowdhury, R., Li, X. S., King, O. N., Lejeune, C., Ng, S. S., Krojer, T., Chan, M. C., Rydzik, A. M., Hopkinson, R. J., Che, K. H., Daniel, M., Strain-Damerell, C., Gileadi, C., Kochan, G., Leung, I. K., Dunford, J., Yeoh, K. K., Ratcliffe, P. J., Burgess-Brown, N., von Delft, F., Muller, S., Marsden, B., Brennan, P. E., McDonough, M. A., Oppermann, U., Klose, R. J., Schofield, C. J. and Kawamura, A. (2012) Plant growth regulator daminozide is a selective inhibitor of human KDM2/7 histone demethylases. J Med Chem. 55, 6639-6643
2012 Lab retreat in Tarbena, Spain with the Brockdorff and Fisher labs
Easter Egg Hunt 2012
Bio-CAP: a versatile and highly sensitive technique to purify and characterise regions of non-methylated DNA
Blackledge, N. P., Long, H. K., Zhou, J. C., Kriaucionis, S., Patient, R. and Klose, R. J. (2012) Bio-CAP: a versatile and highly sensitive technique to purify and characterise regions of non-methylated DNA. Nucleic Acids Res. 40, e32
Christmas Dinner 2011 – Brasserie Blanc
Recognition of CpG Island Chromatin by KDM2A Requires Direct and Specific Interaction with Linker DNA
Zhou, J. C., Blackledge, N. P., Farcas, A. M. andKlose, R. J. (2012) Recognition of CpG island chromatin by KDM2A requires direct and specific interaction with linker DNA. Mol Cell Biol. 32, 479-489
The oncometabolite 2-hydroxyglutarate inhibits histone lysine demethylases
Chowdhury, R., Yeoh, K. K., Tian, Y. M., Hillringhaus, L., Bagg, E. A., Rose, N. R., Leung, I. K., Li, X. S., Woon, E. C., Yang, M., McDonough, M. A., King, O. N., Clifton, I. J., Klose, R. J., Claridge, T. D., Ratcliffe, P. J., Schofield, C. J. and Kawamura, A. (2011) The oncometabolite 2-hydroxyglutarate inhibits histone lysine demethylases. EMBO Rep. 12, 463-469
Quantitative High-Throughput Screening Identifies 8-Hydroxyquinolines as Cell-Active Histone Demethylase Inhibitors
King, O. N., Li, X. S., Sakurai, M., Kawamura, A., Rose, N. R., Ng, S. S., Quinn, A. M., Rai, G., Mott, B. T., Beswick, P., Klose, R. J., Oppermann, U., Jadhav, A., Heightman, T. D., Maloney, D. J., Schofield, C. J. and Simeonov, A. (2010) Quantitative high-throughput screening identifies 8-hydroxyquinolines as cell-active histone demethylase inhibitors. PLoS One. 5, e15535
http://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0015535
Jarid2 is a PRC2 component in embryonic stem cells required for multi-lineage differentiation and recruitment of PRC1 and RNA Polymerase II to developmental regulators
Landeira, D., Sauer, S., Poot, R., Dvorkina, M., Mazzarella, L., Jorgensen, H. F., Pereira, C. F., Leleu, M., Piccolo, F. M., Spivakov, M., Brookes, E., Pombo, A., Fisher, C., Skarnes, W. C., Snoek, T., Bezstarosti, K., Demmers, J., Klose, R. J., Casanova, M., Tavares, L., Brockdorff, N., Merkenschlager, M. and Fisher, A. G. (2010) Jarid2 is a PRC2 component in embryonic stem cells required for multi-lineage differentiation and recruitment of PRC1 and RNA Polymerase II to developmental regulators. Nat Cell Biol. 12, 618-624
CpG islands recruit a histone H3 lysine 36 demethylase
Blackledge, N. P., Zhou, J. C., Tolstorukov, M. Y., Farcas, A. M., Park, P. J. and Klose, R. J. (2010) CpG islands recruit a histone H3 lysine 36 demethylase. Mol Cell. 38, 179-190
http://www.sciencedirect.com/science/article/pii/S1097276510002868
Histone lysine methylation: an epigenetic modification?
Blackledge, N. P. and Klose, R. J. (2010) Histone lysine methylation: an epigenetic modification? Epigenomics. 2, 151-161
PHF8, a gene associated with cleft lip/palate and mental retardation, encodes for an Nε-dimethyl lysine demethylase
Loenarz, C., Ge, W., Coleman, M. L., Rose, N. R., Cooper, C. D., Klose, R. J., Ratcliffe, P. J. and Schofield, C. J. (2010) PHF8, a gene associated with cleft lip/palate and mental retardation, encodes for an Nepsilon-dimethyl lysine demethylase. Hum Mol Genet. 19, 217-222
Phosphorylation of MeCP2 at Serine 80 regulates its chromatin association and neurological function
Tao, J., Hu, K., Chang, Q., Wu, H., Sherman, N. E., Martinowich, K., Klose, R. J., Schanen, C., Jaenisch, R., Wang, W. and Sun, Y. E. (2009) Phosphorylation of MeCP2 at Serine 80 regulates its chromatin association and neurological function.Proc Natl Acad Sci U S A. 106, 4882-4887